It's one of Sheffield's biggest and busiest roads, stretching all the way from the edge of Kelham Island, at Shalesmoor, to Hillsborough Stadium and beyond.
These photos of Penistone Road and surrounding streets from the 1940s, 50s, 60s and 70s show how it has been transformed over time.
Lost pubs, old schools and trams, from the days when Sheffield had a much more extensive network, all feature in this retro photo gallery. The shops pictured along Penistone Road are also bound to bring back memories for many of you.
The images show Sheffield at work and at leisure, with old factories pictured alongside pigeon lofts and Speedway bikers on the track at Owlerton Stadium.
All the photos are shared courtesy of Picture Sheffield.
13. Travellers Inn
The Travellers Inn, on Penistone Road North, Sheffield. A coaching inn where the large fireplace in the kitchen bears the inscription 1697. It is pictured here some time during the 1940s or 50s. Photo: Picture Sheffield/S Cooper | Picture Sheffield/S Cooper
14. Old Crown Inn
The Old Crown Inn, on Penistone Road, Sheffield, an old coaching house, with a blacksmith's shop alongside. When this photo was taken in the 1940s or 50s, the pub was owned by Hope and Anchor Brewery and occupied by Mr R.W. Lambert. Photo: Picture Sheffield/S Cooper | Picture Sheffield/S Cooper
15. Leppings Lane junction
The junction of Leppings Lane and Penistone Road North, Sheffield, in June 1966. Photo: Picture Sheffield | Picture Sheffield
16. 1921
Penistone Road, Sheffield, viewed from Clay Wheels Lane, in 1921 | Picture Sheffield Photo: Picture Sheffield