It's one of Sheffield's biggest and busiest roads, stretching all the way from the edge of Kelham Island, at Shalesmoor, to Hillsborough Stadium and beyond.
These photos of Penistone Road and surrounding streets from the 1940s, 50s, 60s and 70s show how it has been transformed over time.
Lost pubs, old schools and trams, from the days when Sheffield had a much more extensive network, all feature in this retro photo gallery. The shops pictured along Penistone Road are also bound to bring back memories for many of you.
The images show Sheffield at work and at leisure, with old factories pictured alongside pigeon lofts and Speedway bikers on the track at Owlerton Stadium.
All the photos are shared courtesy of Picture Sheffield.
17. 1960s shops
Penistone Road, Sheffield, near the junction with Upper Slack, in March 1963. Pictured are the newsagents Achibold Mc.Gibbon and other businesses. Photo: Picture Sheffield/Sheffield Newspapers Ltd | Picture Sheffield/Sheffield Newspapers Ltd
18. Tool Works
Penistone Road, Sheffield, seen from the junction with Bamforth Street, in March 1963, showing J. H. Swift and Sons Ltd, Dreadnought Tool Works. Photo: Picture Sheffield/Sheffield Newspapers | Picture Sheffield/Sheffield Newspapers
19. Former Army Hotel
J. Lockwood Edwards Ltd, the former Army Hotel, at Hillfoot, Penistone Road, Sheffield, in March 1969. Photo: Picture Sheffield/Mr. Brightman | Picture Sheffield/Mr. Brightman
20. Beres pork sandwich shop
Penistone Road, Sheffield, in February 1973, showing (left) Beres pork butchers and a drapery store, and (right) a fish and chip shop. Photo: Picture Sheffield/Sheffield Newspapers Ltd | Picture Sheffield/Sheffield Newspapers Ltd