It's one of Sheffield's biggest and busiest roads, stretching all the way from the edge of Kelham Island, at Shalesmoor, to Hillsborough Stadium and beyond.
These photos of Penistone Road and surrounding streets from the 1940s, 50s, 60s and 70s show how it has been transformed over time.
Lost pubs, old schools and trams, from the days when Sheffield had a much more extensive network, all feature in this retro photo gallery. The shops pictured along Penistone Road are also bound to bring back memories for many of you.
The images show Sheffield at work and at leisure, with old factories pictured alongside pigeon lofts and Speedway bikers on the track at Owlerton Stadium.
All the photos are shared courtesy of Picture Sheffield.
9. Parkside Road junction
The junction of Penistone Road and Parkside Road in Hillsborough, Sheffield, in March 1959. Photo: Picture Sheffield | Picture Sheffield
10. Owlerton Tram
Passengers boarding Tram No. 150 to Owlerton via Penistone Road, at the Parkside Road Terminus, with Hillsborough School on the left. Photo: Picture Sheffield | Picture Sheffield
11. Hillsborough Park entrance
Penistone Road, Sheffield, showing the entrance to Hillsborough Park and the junction with Parkside Road, some time between 1940 and 1959. Photo: Picture Sheffield | Picture Sheffield
12. Gymnastics
Boys gymnastics at Wadsley Bridge Council School, on Penistone Road North, Sheffield, during the 1940s or 50s. Photo: Picture Sheffield | Picture Sheffield