It's one of Sheffield's biggest and busiest roads, stretching all the way from the edge of Kelham Island, at Shalesmoor, to Hillsborough Stadium and beyond.
These photos of Penistone Road and surrounding streets from the 1940s, 50s, 60s and 70s show how it has been transformed over time.
Lost pubs, old schools and trams, from the days when Sheffield had a much more extensive network, all feature in this retro photo gallery. The shops pictured along Penistone Road are also bound to bring back memories for many of you.
The images show Sheffield at work and at leisure, with old factories pictured alongside pigeon lofts and Speedway bikers on the track at Owlerton Stadium.
All the photos are shared courtesy of Picture Sheffield.
5. Gate Inn
The Gate Inn, on Penistone Road North, Sheffield, in June 1963. Photo: Picture Sheffield/Sheffield Newspapers | Picture Sheffield/Sheffield Newspapers
6. Shalesmoor junction
Penistone Road, Sheffield at the junction with Cornish Street and Shalesmoor, taken from Infirmary Road, in January 1955. The New Inn and the post office can be seen. Photo: Picture Sheffield/Press Photo Agency | Picture Sheffield/Press Photo Agency
7. Speedway
Speedway at Owlerton Stadium, on Penistone Road, Sheffield, some time between 1960-1979. Photo: Picture Sheffield | Picture Sheffield
8. School crossing
Boys from Wadsley Bridge Council School crossing Penistone Road North, in Sheffield, with the Gate Inn and the sign for the Travellers Inn in the background. This photo was taken some time between 1940 and 1959. Photo: Picture Sheffield | Picture Sheffield