The men are believed to hold vital information about a mass brawl in Sheffield city centre which broke out at around 3am on Wednesday, December 22.
South Yorkshire Police said that a group of around 20 people left the Viper Rooms nightclub and violence flared shortly afterwards on the Carver Street car park.
A number of victims were kicked, punched and stamped on during the incident.
Arrests have been made over the brawl but officers are keen to trace a number of people who may be able to assist with enquiries.
The force said: “It is reported that on Wednesday, December 22 at around 3am, a large group of around 20 people have left the Viper Rooms nightclub and walked up to the top of Carver Street, where an altercation has taken place in the Carver Street car park.
“It is alleged that during the altercation, a number of victims were kicked, punched and stamped on.
“Officers have already made a number of arrests in relation to the incident but officers are keen to identify the seven men in the images as they may be able to assist with enquiries. Officers are also keen to speak with anyone that may have witnessed the disorder, or filmed the events on a mobile device.
“Did you witness this incident? Or do you recognise these men?”
Anyone with information should call South Yorkshire Police on 101 and quote incident number 100 of December 22.
Crimestoppers can also be contacted, anonymously, on 0800 555 111.