Dad-of-three Anthony Sumner, 42, was critically injured in an attack on Windy House Lane just before midnight on Thursday, July 29.
Anthony died at the scene from stab wounds, and a 49-year-old man and a 15-year-old boy were later arrested on suspicion of murder.
Richard Sampson, 49, of Fretson Court, Manor, has since been charged and is due at Sheffield Magistrates’ Court today.
The teenager has been bailed pending further enquiries.
This latest stabbing comes just three months after Danny Irons, 32, was murdered, not far away on Fretson Green in April.
Local residents have said that it is ‘scary’ that stabbings are becoming more frequent on the city estate.
One resident, who only wished to be known as Dawn, said that police officers need to be in the area more to prevent crime rather than to just respond to it afterwards.
She said: “More police would probably help. It would be good to see more patrols round here.
“At the moment they only seem to come if there’s something already going off. I understand that there is a shortage of people at the police but there has been two stabbing round here in recent weeks already.
“It’s scary. Extra police are needed.”
Police and council officials are planning to meet in the wake of the latest knife attack.
Fran Perara, who runs Manor Post Office, said that people had been coming in and talking about how they were scared because of how close together the two stabbings happened.
"The other one only happened three months ago,” she said. “It is so sad. It has affected the people here.
“It is so scary that two happened in a few months.”
And another local resident, who wished not to be named, said: Something more needs to be done.
“A man has died not long after another one died in April and there was another stabbing in July as well.”
Floral tributes have been placed on Windy House Lane following the latest murder, where wellwishers paid their respects.
A friend called Tina wrote: “Gone too soon pal, you will be sadly missed.”
Josie and Ash said: “Forever in our hearts and thoughts, love and miss you forever.”
Another note reads: “To Dad, RIP, love Morgan and the kids.”
The police investigation into the death of Mr Sumner is ongoing, and officers have appealed for witnesses.
Det Ch Insp Simon Palmer said: “We are doing everything we can to establish the sequence of events that led to the tragic death of Anthony.
“We know there may have been people in the area who witnessed the incident, but have yet to come forward with information.
“You might think your input is insignificant, but any information you have could be crucial for our investigation.”
Anyone with information should call South Yorkshire Police on 101 or Crimestoppers on 0800 555111.
Quote incident 1,000 of July 29.