Right-back Moses Odubajo and versatile midfielder Adam Reach missed Saturday's draw to relegation-threatened Barnsley due to hamstring and toe injuries respectively.
Odubajo's knock meant Liam Palmer deputised at left-back, with Dominic Iorfa switching from centre-half to the right hand side of their defence, while debutant Josh Windass was given the nod in attack in the absence of Reach.
"They both picked up injuries in the week," confirmed boss Garry Monk, who remains "very hopeful" Morgan Fox (ankle) and Massimo Luongo (groin) will be fit to face Luton at Kenilworth Road on Wednesday evening.
"Reachy (Adam Reach) had a toe problem and Mo has a tight hamstring.
"They will be sent for scans and we will see what the outcome of them are.
"Until we have had the scans and know the full side of it, I can't say a time frame. Hopefully it is not too long."
Wednesday stayed in 11th position in the Championship standings after their Oakwell stalemate, six points adrift of the play-off positions with 15 matches remaining.
Monk admitted: "We knew it was going to be a battle. I think for both teams, being a local derby, it was a fight to win that battle.
"The competitive side was there, but both teams didn't show that care on the ball. The times we did that, we looked a threat, but it just let us down today.”