Stocksbridge Park Steels succumbed to a 1-0 defeat at Marine.
The goal that separated the two teams came in the 72nd minute with a goal for Marine debutant Adam Fowler who made no mistake from 15 yards.
Ironically the goal came just a minute after Steels had created their best chance. A surging run from Jack Muldoon left defenders flatfooted only for the strikers to fire inches over the crossbar.
Worksop Town were well beaten 3-0 at Kendal Town. Danny Williams, an own goal from James Cotterill and a second half strike from Mark Jackson condemned the visitors to defeat.
Matlock Town also crashed to a 3-0 defeat Ashton United.
Chorley v Frickley Athletic, Hednesford Town v Buxton, Stocksbridge Park Steels v Ashton United.
Kidsgrove Athletic v Sheffield FC.
Matlock Town v Chasetown, Worksop Town v Stafford Rangers.