Chesterfield’s re-formed reserves team will compete in the East Division of the Final Third Development League next season.
Their eight-team league will also feature Doncaster Rovers, Gateshead, Hartlepool United, Notts County, Rotherham United, Scunthorpe United and Grimsby Town.
It’s likely that a number of home games will be played at the Proact Stadium.
Academy manager Mark Smith welcomed the club’s re-entry into the formal second-11 arena.
He said: “Keeping players that need development time at home rather than loaning them out is a big advantage. You can work with them all week in the build-up to a game and you’ll know they’ll play in a position and a style that suits the club.
“It also allows the youth-team players the opportunity to play against experienced men, and that speeds up their development significantly.
“Young professionals like Laurence Maguire and Jake Beesley, who came through the youth set-up, together with others like Rai Simons, Mani Dieseruvwe and Michael Onovwigun, will also benefit from the reserves, with regular fixtures guaranteeing regular games against decent players on good pitches.”