Shaw Lane AFC, the Barnsley-based non-league side, have announced they are withdrawing from all football matters with immediate effect.
The Ducks finished seventh in the Evo-Stik Premier Division (seventh tier of English football) last season.
The club put out a statement on Friday afternoon saying owner Craig Wood "feels that he has taken the club as far as he could and was seeking fresh investment to take the club to the next level".
The statement added that: "This was not forthcoming within the timescales necessary therefore; exploratory talks had taken place with a Wakefield based consortium, which again could not provide the required guarantees necessary for the club to compete in step three of the football league pyramid."
Wood said: "The concept of Shaw Lane was always a gamble, which unfortunately has come as far as it can.
"We set out a five-year plan back in 2012, which was to be knocking on the door of the National League and to play a Football League side in the FA cup.
"With great pride, we have achieved this and it would have been a fairytale to be able to take the club even further."