Ten-man Barnsley picked up a valuable three points at Scunthorpe thanks to Josh Scowen’s goal early in the second half.
Reds caretaker bosses Mark Burton and Paul Heckingbottom made two changes from the side that beat Crewe as they looked for more of the same against Scunthorpe.
In came George Waring and on loan John O’Sullivan for Leroy Lita and Kane Hemmings as Barnsley prepare to face former boss Mark Robins.
And after an early scare when Paddy Madden had a shot saved by Adam Davies the Reds nearly went in front through a Conor Hourihane free kick which just went wide.
However, it was the hosts who had the better chances during the first half an hour with Madden and Kevin van Veen both going close.
Liam O’Neil and Hourihane exchanged long range attempts and Barnsley appealing in vain for a penalty as George Waring went down under a challenge from Andrew Boyce.
Waring could have given Barnsley the lead but couldn’t convert Hourihane’s pass before O’Neil saw his effort fly over the bar.
Six minutes after the restart Scowen slotted home to give the Reds the advantage.
Burton’s team were reduced to ten men before the hour as defender Jean-Yves Mvoto picked up a second yellow card.
Scowen almost doubled Barnsley’s lead but was wide and then Davies did well to deal with a Boyce header.
The Iron pressed for an equaliser but couldn’t find a way past a resolute Reds defence.
Scunthorpe: Daniels, Clarke, Boyce, Canavan, Williams, Bishop, O’Neil, Osbourne, Madden, Hopper, van Veen. Subs: Anyon, Nolan, Evans, Adelakun, McSheffrey, Wootton, Llera.
Barnsley: Davies, Cranie, Nyatanga, M’Voto, Smith, O’Sullivan, Hourihane, Pearson, Scowen, Bailey, Waring. Subs: Dibble. Berry, Lita, Hemmings, Holgate, Lalkovic, Oates