YORKSHIRE Wildlife Trust has called for mitigation work to minimise the impact of the high speed railway on nature reserves.
Sheffield and Rotherham Wildlife Trust fears 12 natural habitats could be threatened by the line, which will run through Killamarsh, near Beighton and Woodhouse, Tinsley, Wincobank and north close to Thorpe Hesley.
One of the sites, Woodhouse Washlands, is among three regionally-important nature reserves which Yorkshire Wildlife Trust is keen to protect.
Dr Rob Stoneman, chief executive of Yorkshire Wildlife Trust, said: “We are extremely concerned that, if poorly planned and designed, high speed rail could create a very expensive swathe of concrete and steel through the beautiful countryside, fragmenting our diminishing wildlife yet further.
“We are very concerned about damage to our reserves and other wildlife sites along the route, especially in south Sheffield at Woodhouse Washlands Nature Reserve.”
He said ‘a very small part of the massive HS2 budget’ should be put aside to improve wildlife habitats around the line.