Buses will replace trams while maintenance is carried out on the Sheffield Supertram network.
Blue and purple routes will not be able to operate between Gleadless Townend and Sheffield railway station all day on Friday, November 2.
The blue route will run between Halfway and Gleadless Townend as well as Sheffield railway station and Malin Bridge.
There will be no purple route services all day.
A replacement bus will run from Herdings Park to Fitzalan Square, serving all tram stops on the way and connecting with blue trams at Gleadless Townend and Fitzalan Square.
Trams between Halfway and Gleadless Townend and the replacement bus route will operate every 15 minutes most of the day and every 20 minutes in the evening.
In a statement, operator Stagecoach Supertram advised people to allow up to 20 minutes extra time for their journeys.
Tram tickets will be valid on all Stagecoach services between Halfway and the City Centre, including 120.
Yellow route and tram-train services will operate as normal.
Trams will return to normal from the start of service on Saturday, November 3.
The statement added the works couldn’t be carried out overnight and required a ‘full-day possession’ of the tracks.
It said: “We apologise for any disruption this may cause.”