Burngreave Road is closed near Pitsmoor Surgery, Pitsmoor, while emergency services deal with the incident.
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Bus company First South Yorkshire said its services which use Burnrgeave Road - 1a, 20, 75 and 76 - have been diverted via Scott Road and Ellesmere Road and are resuming their normal routes at Gower Street due to a '˜police incident'.
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South Yorkshire Police said there has been a collision but no other details have yet been released.