Season ticket-holder Michael Witheford died on January 2 and his family launched a campaign for fans to pay tribute to him during the derby.
A message on Twitter asked both sets of fans to join together for a minute of applause on the hour-mark to honour Michael's life.
The message read: "My lovely friend sadly lost her husband, Michael Witheford, on January 2 2018. He was a lifelong Blades fan and season ticket holder.
"I would like to ask if you are attending the Sheff Utd v Sheff Wed match on Friday if you could kindly give a round of applause on the 60th minute (Michael's age).
"His family would be overwhelmed if we could make this happen and I am sure Michael will be there in spirit too. Thank you!"
Sheffield United confirmed, via their commercial Twitter account, that Michael would indeed be honoured during the derby.
They tweeted: "Hi All - We are advised that Michael will be acknowledged in the programme and on the big screen on Friday.
"Michael was a TC10 Executive Suite member and will be sadly missed."