The collision at Malin Bridge has resulted in a number of buses being diverted.
First South Yorkshire tweeted at around 10.30am to say its 52a, 81 and 82 bus services were all affected by the collision, which it clarified did not involve a bus.
The opertor said the 52a was being diverted via Middlewood road, Wadsley Lane and Far Lane before rejoining its normal route at the junction of Dykes Hall Road.
It said number 81 and 82 buses would be terminating at Hillsborough until Malin Bridge reopened.
The road has since reopened.