A police 'day of action' on Sunday saw officers carrying out patrols between Stainton in Doncaster and Maltby in Rotherham.
They seized bikes from four riders who had previously been warned about using them in an anti-social manner.
Inspector Mark Payling, of South Yorkshire Police, said: “We know that people in the local area have concerns about off-road bikes and I want to reassure residents that we are taking action.
“Bikers can be dealt with for not wearing helmets, riding a bike without a valid licence or MOT, as well as other road safety offences. I don’t want people to suffer in silence if these bikes are bothering them.
“Information we receive from members of the public is really important in helping us to clamp down on this issue, particularly as bikers are often wearing helmets, which can make it hard to identify them.
The operation is to be repeated.
:“The operation will be extended across the district to ensure that off-road bikes are not causing problems within the community," added Insp Payling.
“Anyone who is caught riding an off-road bike without a helmet, in an area where they shouldn’t be or without the necessary documents, will have their bike seized and could face being issued with an antisocial behaviour notice.”