David Haggerty 29, said he and three of his friends are raising funds for Create A Dream Foundation, something that is close to their hearts.
The challenge, which will begin on June 24 and end on June 26 at Edinburgh Castle, will see them travel along the coastline in the final part of their journey.
David, who works as a health and safety consultant in the city said: "We wanted a challenge that would push ourselves to the limit and we felt Edinburgh in three days would do this.
"We are excited to ride along the coast for the final part of our journey as the views we've been told are breathtaking.
"Edinburgh Castle, being our finish line where we are hoping our friends and family will be able to join us."
He will be joined by his friends, a company director Danny Leesley, 31, supply chain analyst Brad Addinall, 27 and Alan Kay, 63, who works as delivery driver.
David said the chose the charity as it was "close to home" and local.
"A mutual friend told us about Create A Dream and we couldn't think of a more fitting charity.
"The majority of us have young children or grandchildren and we could not imagine being in the position these families are in, so anything we can do to help is brilliant.
"We know whatever we raise will give a child something to look forward to and smile about," he said.
And cycling for hundreds of miles is no easy feat, said David, as they have to prepare well ahead of the challenge.
He said: "Every weekend, we have been out on our bikes with both short and long distance rides.
"Danny and I completed our first 100-mile ride to Lincoln and back at the start of January.
"Danny and Brad have got indoor cycling trainers which helps a lot when the weather isn't so great. Alan has been a life-long cyclist and at the age of 63, is still going strong.”
Those who would like to support their fundraising campaign, please visit here.