Members of Network Rail’s safety team used specialist cleaning equipment to rid vandalism from a wall behind the station and next to the Supertram lines.
It has been repeatedly tarnished with graffiti over recent months and has been seen by thousands of commuters using the tram and railway station daily.
Network Rail, which maintains the UK’s railways, decided to take action following a series of calls from passengers and residents urging them to clean up the area.
As part of the day of action, the safety team agreed to hold their monthly meeting at the site instead of in the office so they could clean off the paint.
Hayley Bull, community safety manager, said: “Graffiti is a problem on the railway and unfortunately this area near Sheffield station had become particularly bad.
“We decided to have our team meeting outside the normal office environment which meant we were still able to have all the conversations we needed to, while doing something that improves this part of the railway for those who live, work and visit Sheffield.”
Network Rail spends more than £3.5 million a year on cleaning graffiti up from railway structures across the country. A spokesperson also warned those that trespass on the railway to spray paint are putting themselves and others in great danger.