The event will take place at St Hugh of Lincoln Church at 135 Littlemoor, Newbold, from 7pm on Monday.
Speakers will be former MP and broadcaster George Galloway, Alex Gordon, of the RMT, Brendan Chilton, from the Labour Party, Mark Hill, of the Green Party, and a speaker from the Indian Workers’ Association.
Labour Leave activist Gary Matthews claimed Chesterfield library had originally agreed to hold the event but had now cancelled it for political reasons.
Library bosses insist they do not have a record of a booking for Mr Matthews’ use of the lecture theatre.
Mr Matthews said: “I feel like we were been banned from holding this event at the library because Derbyshire County Council – which runs it – is Labour-led and pro-EU.
“At least we’ve found another venue for the rally.
“Everyone is welcome to attend. Bring on a good debate.”
Members of the public will go to the polls next Thursday in an historic referendum to decide whether Britain should leave or remain in the EU.
Anti-EU campaigners believe Britain is being held back by the organisation, which they say imposes too many rules on business and charges billions of pounds a year in membership fees for little in return. They also want Britain to take back full control of its borders.
Those campaigning for Britain to stay in the EU say it gets a big boost from membership – it makes selling things to other EU countries easier and, they argue, the flow of immigrants, most of whom are young and keen to work, fuels economic growth and helps pay for public services.
For more information about the rally, call Gary on 01246 270513.