Officers dressed in protective white suits are carrying out a forensic sweep of a crime scene cordoned off at the junction of Nodder Road and Hastilar Road South, Woodthorpe, following the shooting at 9.10pm yesterday.
The crime scene, which also takes in part of Fishponds Road West and Chadwick Road, is under police guard this morning.
South Yorkshire Police said the shooting victim was taken to hospital, where he remains today.
His injuries are not believed to be life threatening.
The shooting was the third in that area in the space of three weeks.
On Tuesday, May 8, a 30-year-old man was shot in his leg in Chadwick Road in broad daylight.
The shooting happened close to Woodthorpe Community Primary School.
Three weeks earlier a 42-year-old man was shot in his leg in Nodder Road.
Anyone with information about the latest incident should call South Yorkshire Police on 101 and quote incident number 989 of June 11.