James Hudson Taylor was born in Barnsley in 1832 and went to China, founding the China Inland Mission, which is one of the largest Christian movements in the world.
In May 2012 the original Hudson Taylor Trail was officially opened, featuring 14 plaques around Barnsley, marking historical sites.
The plaque at the Sixth Form College, originally on the Central Library, marks the site of the White Bear, where Anglican minister John Wesley, credited with founding the Methodist movement, preached to the people of Barnsley.
The plaque was removed from the library to allow the £18 million Sixth Form College, which opened in January, to be constructed.
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Members of the James Hudson Taylor Group, Barnsley College Principal Chris Webb and Barnsley Sixth Form College Principal Liz Leek attended the installation ceremony.
James Hudson Taylor Group member Michael Rossiter said: “We hope that the trail will bring people from China and other parts of the world to Barnsley.”