The ex-England footballer has been refused permission to appeal against his conviction for sex acts with a girl aged 15.
Johnson, 28, was jailed for six years after being found guilty of sexual touching, but not guilty on a charge relating to another sexual act.
He is currently serving his sentence at Doncaster's Moorland Prison.
The former Sunderland player had previously admitted grooming the girl and one charge of sexual activity.
The disgraced winger’s sister Faye posted a message on Twitter, saying: “Absolutely devastated that the single judge has rejected my brother’s appeal against sentence and conviction.
“But I can assure every supporter that it has made me stronger and I will never give up.”
The player's trial at Bradford Crown Court heard that he had kissed and sexually touched the girl in his Range Rover, in a secluded spot in County Durham.
A statement from his solicitor said: "We have received notification that Adam Johnson's applications for leave to appeal against his conviction and sentence have been refused by the Single Judge.
"He is obviously disappointed - having carefully considered all of the information, we will be lodging renewed applications on conviction and sentence in the coming days."