The race weaves its way through Derbyshire via Buxton, Whaley Bridge, Chinley, Chapel-en-le-Frith, Hope Valley, Grindleford, Stoney Middleton, Wardlow, Bakewell, Chatsworth, Beeley Moor, Matlock, Cromford, Carsington Reservoir, Belper and Ilkeston, on Friday 11 September.
Riders are expected to be in the county between around 11am and 3.40pm before heading to a finish in Nottingham during Stage 6 of the event, which starts in Stoke-on-Trent.
Rolling road closures will be in place in advance of the race and motorists are asked to avoid parking on the race route during these approximate times:
High Peak area 10am to 1.30pm
Sheffield roads with speed cameras including Parkway, Manor and Crosspool
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Derbyshire Dales area 11am to 2.45pm
Amber Valley area 12.45pm to 3.30pm
Erewash area 1.15pm to 3.45pm
And businesses are advised not to arrange large deliveries in the lead up to the race.
Highways officials are also warning road users there will inevitably be some delays on the race route between late morning and mid-afternoon and while the roads are cleared post-race.
These could be between 20 and 40 minutes depending on how busy the roads are.
No Parking notices will make it clear where vehicles should not be parked.
The police will be able to move cars that are inappropriately parked.
In some areas parking will be limited and spectators are urged to walk, cycle or take the bus, if possible.
Timings and the race route showing affected roads can be viewed at www.tourofbritain.co.uk
Councillor John Owen, Deputy Cabinet Member for Highways, Transport and Infrastructure, said: “Derbyshire is leading the way in building a reputation as a top cycling destination so we are pleased to be hosting a leg of this prestigious cycling event.
“We want people to enjoy themselves but we expect it will be very busy. Inevitably there will be some delays on the roads to and from the race route but for everyone’s safety we ask that people are patient when travelling and park appropriately.”
Go to www.derbyshire.gov.uk/tour for road closure details.