Dam Health UK has launched sites in Woodhouse Pharmacy and in Wincobank creating 10 jobs. It promises same day results for £99.
Boss, Prof Frank Joseph, believes regular testing is here for the long term and will be needed for overseas holidays or work trips. People are also taking tests before visiting extended family.
He added: "Until we know whether the vaccine stops us from transmitting the virus, we will still need to be tested on a regular basis. Previous vaccines for other things have shown a decrease in transmissibility, but we can't take anything as a given with this virus, so we need to continue to work towards protecting people and this includes testing.
"People are already making booking enquiries with us ahead of the summer holidays when travel rules could be relaxed."
DAM Health UK launched its first testing clinics in Liverpool and Manchester in December.