Sheffield United, the lowest ranked team left in the FA Cup, are through to the semi-finals of the tournament after second-half goals by Ryan Flynn and John Brayford.
Clough, who won the League One manager of the month award for February, has opted to make seven changes to his starting line-up.
Centre back Harry Maguire (knee), Jamie Murphy (groin) and John Brayford (calf) have shrugged off their injuries and been recalled. Bob Harris, Ryan Flynn, Jose Baxter and Stefan Scougall also return.
United: Howard; Brayford, Collins, Maguire, Harris; Flynn, Doyle, Coady, Scougall, Murphy; Baxter, Substitutes: Hill, Porter, Miller, McGinn, Davies, Long, Freeman.
Charlton: Hamer; Wilson, Wiggins, Morrison, Wood; Cousins, Poyet, Jackson, Harriott,; Tudgay, Church. Substitutes: Thuram-Ulien, Hughes, Dervite, Green, Ghoochannejhad, Nego, Ajdarević.