Ritchie Humphries and Armand Gnanduillet could feature for Spireites reserves this afternoon at Scunthorpe United.
The duo, along with the recently returned from loan Aaron Chapman, are part of a 15-man squad taking on the Iron.
The Final Third Development League Cup tie will kick off at 2pm at Scunthorpe’s Glanford Park.
Spireites confirmed the squad as the following on their official website earlier today: Aaron Chapman, Laurence Maguire, Ritchie Humphreys, Dion Donohue, Jake Orrell, Michael Onovwigun, Armand Gnanduillet, Rai Simons, Byron Harrison, Jake Beesley, George Milner, Derek Daly, Tom Marshall, Dylan Parkin and Triallist.
Spireites will also take on Notts County and Rotherham United in the group stages of the competition.