Spireites utility man Drew Talbot is out of the reckoning for selection for up to six weeks.
The former Owls striker turned right back (pictured below) has been having problems with a persistent groin injury for many months now.
The 27-year-old, who hass been at the club since 2009, played less than an hour in the final three months of last season but, despite making a brief appearance at Matlock Town in last week’s pre-season game, his problem has not gone away.
Head of sports science Rodger Wylde said: “This injury is a manifestation of problems in the groin area over the last 18 months.
“They’ve unravelled each problem one-by-one and now hopefully this will be the last piece of the jigsaw on the road to recovery.”
Talbot tweeted: “So bad news it is! No time to sulk and feel sorry for myself, I’ll be working harder than ever, if that’s possible, to get myself back fit!”
Chesterfield’s first team travel to Conference National side Lincoln City this afternoon, fresh from a 5-1 win at fellow level five side Alfreton Town on Tuesday.
Goalkeeper coach Eric Nixon is hoping Tommy Lee will be fit to play his first pre-season game after a minor injury while Liam Cooper, Jay O’Shea and Eoin Doyle will all be assessed after niggles picked up in the week.
French-born striker Armand Gnanduillet will be hoping to carry on his solid preseason form. He scored at Matlock and added three more at Alfreton in midweek.
Trialists Nicky Featherstone and Adam Morgan will be anxious to get another chance to impress manager Paul Cook.
Midfielder Featherstone, a Walsall player for the last two seasons, has looked impressive to date whilst striker Morgan, contracted to Yeovil Town but available on a free transfer, will be desperate to get a goal in his bid to earn a move away from Huish Park.