After a perfect start to November with a hat-trick of victories, the Reds suffered their second successive defeat at the hands of Scunthorpe United.
Striker Kane Hemmings was the man left out, with Fulham loanee Marcello Trotta replacing him in attack.
Australian midfielder Ryan Williams remains sidelined by a minor ankle problem, and Reece Brown replacing Paul Digby on the Barnsley bench was their only other change.
Defeat last weekend ended a run of three straight victories for the Reds, and they faced a Scunthorpe side managed by former boss Mark Robins.
After a 2-2 draw against Crawley last time out, he made just a single change for his return to Oakwell, with Alex Davey replacing Andrew Boyce.
The first effort on goal didn’t come until just short of the half-hour mark, and it came against the run of play.
Scunthorpe’s busy forward Lyle Taylor struck a left-footed shot well over the bar from outside the box and the Reds replied immediately.
Left-back Joe Dudgeon found MARCELLO TROTTA in the area, who justified his position in the starting line-up by smashing the ball into the top corner.
But PADDY MADDEN equalised within a minute with a right-foot shot into the top right-hand corner.
After the Reds’ opener and Scunthorpe’s quick-fire response, the game certainly livened up. Conor Hourihane wasted a good opportunity from a set-piece, shooting high and wide from just outside the box.
Fulham loanee Trotta was eager to add to his goal tally, hitting a left-footed shot straight at the ‘keeper just a moment after heading over the bar.
The home side’s wastefulness was punished just before half-time, as MADDEN struck his second of the match - a low effort beating Turnbull from the edge of the area, giving the visitors the lead going into the break.
Barnsley: Turnbull; Cranie, Nyatanga, Ramage, Dudgeon; Berry, Hourihane, Bailey, Cole; Trotta, Winnall.Subs: Davies, Brown, Treacy, Hemmings, M’voto, Abbott, Lita.
Scunthorpe: Slocombe; Hawkridge, Llera, Davey, Williams; O’Neill, Williams, Bishop, McSheffrey; Madden, Taylor.Subs: Olejnik, McAllister, Sparrow, Myrie-Williams, Adelakun, Kee, Brisley.