Sheffield Friends of the Earth has joined thousands of people in objecting to plans for a motorway service station which would affect ancient woodland.
The group said the plans for Smithy Wood in Chapeltown were not needed.
Member David Roberts added: “Our ancient woodlands are quintessential features of England’s much-loved landscape - irreplaceable, living historic monuments. The government’s own planning rules state planning permission should be refused for development resulting in the loss of ancient woodland.”
A separate inquiry into a bid for a village green at the woods is also to take place but Sheffield Council will not know the cost until an inspector is chosen.