A DNA test carried out last month on a man who believed he was Ben Needham was negative, South Yorkshire Police have revealed.
The force has been exhausting old lines of enquiry into the disappearance of the Sheffield toddler as their fresh investigation gets underway.
It has been working with Greek authorities to tie up loose ends from previous lines of enquiry, as its own probe began this month.
A spokesman said: “As part of those enquiries, a DNA test was carried out in March on a man in Greece who believed he could have been Ben. The DNA test confirmed the man was not Ben Needham.
“The inquiry goes on and the force will continue to support both Ben Needham’s family and the Greek authorities in progressing the investigation and establishing what happened to Ben.”
Sheffield toddler Ben was 21 months old when he disappeared from outside a farmhouse his grandparents were renovating on the Greek island of Kos in 1991.
His mum Kerry, from Parson Cross, has spent years travelling to and from the island following up leads and potential sightings and has handed South Yorkshire Police a dossier of information she would like detectives to look at.
In October 2012 some South Yorkshire officers travelled to Kos to excavate a mound of earth near to where Ben was last seen alive.
They were investigating a theory he may have been accidentally buried under rubble by a JCB driver working in the area at the time.
Last month, the police were awarded Home Office funding to start a new investigation and a team of officers has now been drawn up.
Anyone who has information relevant to the investigation, who has not already reported their information to police, can contact the inquiry team on 0114 2963025 or email them at firstname.lastname@example.org.