Aldi's Meadowhall Retail Park store will officially close next month.
The supermarket giants have confirmed that the store will close on April 30.
The decision to close the store comes following the announcement that the new Aldi store on Drakehouse Way will open on April 6.
An Aldi spokesperson confirmed that all staff would at the Meadowhall site would be transferred over to the new store.
A spokesperson said: "Our Meadowhall store will close on April 30 as we open our new, larger store at Drakehouse Way on April 6, which reflects our increasing popularity with local shoppers.
"Our employees are relocating to work this new store, which will also create a further 15 jobs for the local community, and we look forward to welcoming our existing customers to this store.
"We also plan to open further stores within Sheffield, including at St James Retail Park, Norton."