Campaigners organised the walk to raise awareness about their fight to stop the mass limestone extraction from the prominent Peak District beauty spot.
Led by local ecologist Dr Steve Furness, the walk took in many of the areas believed to be at risk, including a designated Site of Special Scientific Interest at Coombs Dale.
The area features many rare UK species of plants such as orchids, as well as butterflies and lizards.
Malcolm Wootton, of the Save Longstone Edge Campaign, said: "The more people that see the devastation with their own eyes, the better.
"For many it was the first time they had seen what is at risk for themselves.
"All participants left feeling shocked and dismayed, but also much better-informed about what is happening and what is at risk from this large-scale limestone extraction - especially the newly-decimated area of Wager's Flat - prominently visible from Bakewell and Chatsworth."
Another rally is due to be held at 11.30am on Sunday to coincide with an TV broadcast by BBC Look North's Politics Show into the quarrying row.